The flash-small-pool PCR: how to transform blotting and numerous hybridization steps into a simple denatured PCR

PCR, Southern blotting and oocyte expression to assess the efficacy of subtractive hybridization.

Subtractive hybridization is a technique designed to isolate genes and transcripts abundant in or unique to a particular condition. The method has been utilized for constructing a subtracted cDNA library, differential cloning and identifying differentially expressed genes in specific cell lines (3–6,8,9). The analysis of the transcripts isolated by using this technology can be most difficult, i...

the present study is a survey os postmodern historical novel with respect to the voice of women. it argues how the recorded history is regulated by the rules of power and how women have been excluded from this discourse.ther study analysez angela carters nights at the circus as an example of postmodern historical novel to illustrate how this novel has questioned the historical narrative and how it has inserted women into history by breaking the rules of patriarchy.

the present thesis is a study of postmodern historical novel with respect to the voice of women. according to postmodern historiography,the established institution of history is one of the other manifestations of patriarchys power which has silenced women. the study analyzes angela carters nights at the circus as a postmodern historical novel which makes its female characters transgress the pat...

Rapid Detection of Escherichia coli O157: H7 by Fluorescent Amplification–Based Specific Hybridization (FLASH) PCR

BACKGROUND Escherichia coli O157:H7 is an enteric pathogen which can be frequently found asymptomatically in ruminant mammals, but can cause diseases from mild diarrhea to hemolytic uremic syndrome in humans. METHODS We developed fluorescent amplification-based specific hybridization (FLASH-PCR) assay to detect the Stx-encoding gene Stx-1 of E. coli O157:H7. RESULT PCR product of 336 bp was...

How to Transform an Analyzer into a

rapid detection of escherichia coli o157: h7 by fluorescent amplification–based specific hybridization (flash) pcr

background escherichia coli o157:h7 is an enteric pathogen which can be frequently found asymptomatically in ruminant mammals, but can cause diseases from mild diarrhea to hemolytic uremic syndrome in humans. methods we developed fluorescent amplification-based specific hybridization (flash–pcr) assay to detect the stx-encoding gene stx-1 of e. coli o157:h7. result pcr product of 336 bp was suc...